Figure 3.
Snx27 Regulates Junctional Levels of Fmi and Stbm via the PDZ Binding Motif of Fmi
(A and D) 28-hr APF (A) or 32-hr APF (D) pupal wings carrying clones of Snx27 (Figure S2A), marked by loss of GFP immunolabeling (green in A) or RFP fluorescence (red in D). Wings are immunolabeled for Fmi in red and Stbm in blue (A) or Fmi in blue and phalloidin in green (D). Scale bar 10 μm.
(B) Quantitation of mean intensity of Fmi (red dots) or Stbm (orange dots) membrane labeling in pupal wing clones of Snx27. Intensity is shown as a ratio of signal in Snx27 mutant compared to wild-type in each wing.
(C) Mean polarity and variation in polarity angle of wings immunolabeled for Fmi in wild-type and Snx27 mutant tissue.
(E) 28-hr APF pupal wing with twin clones of arm-PRO-EGFP-fmi next to arm-PRO-EGFP-fmiΔPDZ binding motif (ΔPDZbm), marked by β-gal immunolabeling in blue, in a fmiE59/fmiE45 mutant background. The wing is immunolabeled for EGFP in green and Stbm in red.
(F) Quantitation of mean intensity of membrane labeling of EGFP-Fmi (red dots) and Stbm (orange dots). Intensity is shown as a ratio of signal in ΔPDZbm compared to full-length protein in each wing.
(G) Mean polarity and variation in polarity angle of wings immunolabeled for EGFP in EGFP-fmi and EGFP-fmiΔPDZbm tissue.
(H) Quantitation of mean intensity of EGFP-Fmi membrane labeling. Intensity is shown as a ratio of EGFP signal in Snx27 mutant compared to wild-type in each wing.
(I and J) 28-hr APF pupal wings expressing Arm-PRO-EGFP-fmi (I) or Arm-PRO-EGFP-fmiΔPDZ binding motif (ΔPDZbm) (J) and carrying clones of Snx27, marked by loss of RFP (red). EGFP immunolabeling is in green.
(B, F, and H) Error bars are SD. One-sample t tests were used to determine whether the ratio differed from 1.0; ∗∗∗p < 0.001.
(C and G) Values from the same wing are linked by black bars; mean and SD are listed. Paired t tests were used to compare values in the same wing; ∗∗p < 0.01.
See also Figures S2, S3, and S4 and Data S1 for all statistical comparisons.