Fig. 6.
Changes in expression of Lpcats (A) and bile acid transporters (B) by TNF-α and TGF-β1 in primary hepatocytes. Primary hepatocytes were treated with TNF-α (100 ng) and TGF-β1 (10 ng) for 12 and 24 hours, respectively, and harvested to extract total RNA. The mRNA levels were normalized to those of 18S ribosomal mRNA and subsequently normalized to those of vehicle-treated hepatocytes in the same time point. Statistical analysis was performed using one-way ANOVA with Dunnett’s test (n = 3 in each group). *, P < 0.05; **, P < 0.01; ***, P < 0.001.