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. 2019 Feb 11;2(1):e201800186. doi: 10.26508/lsa.201800186

Figure 3. Vamorolone inhibits inflammatory signaling in macrophage and heart cells.

Figure 3.

(A) RAW 264.7 macrophages were pretreated with drug at 10 μM and inflammatory signaling was induced for 24 h using LPS. Expression of NF-κB–regulated inflammatory genes (Il1b and Il6) was assayed by qRT-PCR. (B) IL1B and IL6 protein levels were assayed in media from the same experiment via AlphaLISA assay. (C) Primary cardiomyocytes were pretreated with vehicle, vamorolone, or the GR-specific ligand deflazacort, and inflammatory signaling induced with TNF. NF-κB–regulated inflammatory gene expression (Il1b and Il6) was assayed by qRT-PCR. (D) IL6 protein levels were assayed by AlphaLISA. (E) HL-1cells were pretreated with 10 μM drug and induced with TNF for 24 h. Expression of Il6 was assayed by qRT-PCR. (n = 4, **P < 0.005, ****P < 0.0001, ANOVA with post hoc versus [+] TNF control in gray; [−] = no TNF control, [+] = TNF plus vehicle, D = deflazacort, P = prednisolone, V = vamorolone, E = eplerenone).