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Overlay of the native MS spectra for (A) glycosylated and (B) deglycosylated mAb‐biotins. The 23+ charge state of the parent mAb is highlighted by the blue dashed line. (C) the average masses are calculated and plotted against the number of biotin equivalents reacted with the mAb. The peak width or mass resolution is shown as dashed line. (D) CCS values in nitrogen derived from TWIMS measurements are shown for each charge state of each sample.
