Figure 1.

Rhodopsin–Gi assembly. (A) Two views of the rhodopsin‐Gi protein‐Fab complex structure (human rhodopsin with human Gαi/rat Gβ1/Bovine Gγ2; PDB code: 6CMO). Rhodopsin is colored in dark green, Gαi Ras domain in magenta, Gαi α helical domain (AHD) in red, Gβ in blue, Gγ in yellow, and the Fab in gray. The key structure interface elements of Gαi with rhodopsin, α5 and αN, are labeled.(B) Structure interface between the rhodopsin TM pocket and α5 of Gαi. The key interface residues are labeled; hydrophobic residues in the rhodopsin TM pocket that interact with α5 of Gαi are depicted in gray surface presentation. The same color code is used as in panel A.