Table 1.
Toxicity and Mutagenicity of AR and 5,6-Epoxy-RP with UVA in L5178Y/Tk+/− Mouse Lymphoma Cells
| experiment 1 |
experiment 2 |
|||||
|---|---|---|---|---|---|---|
| treatment | dose (µg/mL) | concn (µM) | mutant frequencya (×10−6) | rel total growth (%) | mutant frequencya (×10−6) | rel total growth (%) |
| control | b | 55 (20/35) | 100 | 96 (24/72) | 100 | |
| AR | 10 | 37.3 | 78 (29/49) | 99 | 119 (32/87) | 93 |
| AR | 25 | 93.3 | 77 (38/39) | 74 | 127 (44/83) | 68 |
| AR + UVAc | 0 | 0 | 153 (63/90) | 77 | 147 (38/109) | 70 |
| AR + UVA | 1 | 3.7 | 212 (106/106) | 50 | 171 (75/96) | 68 |
| AR + UVA | 2.5 | 9.3 | 284 (136/148) | 40 | 212 (104/108) | 50 |
| AR + UVA | 5 | 18.7 | 318 (150/168) | 23 | 261 (140/121) | 33 |
| AR + UVA | 7.5 | 28.0 | NDd | NDd | 396 (220/176) | 23 |
| AR + UVA | 10 | 37.3 | 453 (298/155) | 10 | 448 (294/154) | 11 |
| 4-NQOe | 0.1 | 614 (245/368) | 70 | 611 (278/332) | 69 | |
| control | b | 53 (25/28) | 100 | 101 (33/68) | 100 | |
| 5,6-epoxy-RP | 10 | 18.5 | 45 (21/24) | 95 | 99 (30/69) | 104 |
| 5,6-epoxy-RP | 25 | 46.3 | 61 (28/33) | 95 | 89 (30/59) | 87 |
| 5,6-epoxy-RP +UVAc | 0 | 0 | 141 (69/72) | 68 | 179 (61/118) | 78 |
| 5,6-epoxy-RP +UVA | 1 | 1.9 | 161 (62/99) | 67 | 187 (71/116) | 69 |
| 5,6-epoxy-RP +UVA | 5 | 9.3 | 179 (52/127) | 66 | 197 (80/117) | 72 |
| 5,6-epoxy-RP +UVA | 10 | 18.5 | 184 (91/93) | 70 | 184 (79/105) | 76 |
| 5,6-epoxy-RP +UVA | 15 | 27.8 | 182 (73/109) | 64 | 196 (72/124) | 73 |
| 5,6-epoxy-RP +UVA | 20 | 37.0 | 187 (84/103) | 45 | 239 (96/143) | 55 |
| 5,6-epoxy-RP +UVA | 25 | 46.3 | 237 (108/129) | 39 | 276 (127/149) | 47 |
| 4-NQOe | 0.1 | 599 (256/343) | 59 | 598 (305/293) | 54 | |
a
Numbers in parentheses denote the mutant frequencies for small/large colonies.
b
In the control group, cells were treated with 1% DMSO only.
c
Cells were concomitantly exposed to different concentrations of compounds (AR or 5,6-epoxy-RP) and 1.38 mW/cm2 UVA irradiation for 30 min.
d
Not determined.
e
4-NQO was used as a positive control. Abbreviation: concn, concentration; rel, relative.