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. 2018 Jun 19;41(1):7–16. doi: 10.1016/j.htct.2018.05.001

Figure 2.

Figure 2

Human adipose tissue-derived stem cells culture, viability and characterization. (A) Human mesenchymal stem cells were isolated from adipose tissue in low-glucose Dulbecco's modified eagle medium. The cells formed small colonies within three days of culture as shown by the arrows. The plate showed adherent, spindle-shaped fibroblast-like cells coming out of the colonies. Human mesenchymal stem cells isolated in low-glucose Dulbecco's modified eagle medium reached 80–85% confluency by Day 10 after seeding (magnification 10×). (B) Viability of adipose tissue-derived stem cells after freeze/thaw cycles (n = 5). Forward scatter versus log fluorescence 3 (FL3) channel (7-AAD fluorescence) dot plot shows the mean percentage of viable and dead cells. (C) The adhered mesenchymal stem cells showed spindle-shaped morphology by hematoxylin & eosin staining. Giemsa staining also confirmed the spindle-shaped morphology of adipose tissue-derived stem cells. (D) CD34/45 and HLA-DR expressions were negative, while CD90/CD73/CD105 expression was observed on the cell surface (shown in green). The nucleus was stained with 4′,6-diamidino-2-phenylindole (blue).