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. 2018 Jun 19;41(1):7–16. doi: 10.1016/j.htct.2018.05.001

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© 2018 Associação Brasileira de Hematologia, Hemoterapia e Terapia Celular. Published by Elsevier Editora Ltda.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Trilineage differentiation potential of human adipose tissue-derived stem cells. (A) Phase contrast micrographs showing the different stages of differentiation of human adipose tissue-derived stem cells to adipocytes. Adipocytes with oil droplets are visible on Day 14 of adipogenic induction; the size and number increased as the differentiation progressed. Oil Red O stained the lipid droplets red on Day 21 (magnification 20×). (B) Phase contrast micrographs showing different stages of the differentiation of human adipose tissue-derived stem cells to osteoblasts. Day 14 of osteogenic induction showed visible mineral deposits. There was a significant increase in the mineralization on Day 21 of osteogenic induction (magnification 20×). (C) Pellet culture of human adipose tissue-derived stem cells on Day 21. Hematoxylin & eosin staining showed homogenous cell distribution. Alcian blue staining of acid showed mucopolysaccharide aggregates in the differentiated chondrocytes.