Table 2.

Effect of temperature on the development of RNAi phenotypes

Temperature	Incubation time with dsRNA against Mlig-ddx39 gene until lethalitya, days, mean ± standard deviation		Percentage of animals with enlarged testesb after 4 days of incubation with dsRNA against Mlig-sperm1 gene
	DV1	NL10	NL10
20 °C	17.8 ± 1.3	16.6 ± 1.9	0%
25 °C	10.1 ± 0.6	9.5 ± 0.5	25%
30 °C	7.9 ± 0.2	7.2 ± 0.4	85%

^ano lethality was observed at the tested temperatures when incubating animals with dsRNA against gfp as a control

^bwhen testes touch each other