Table 2.
TAG and PL analysis in DGAT-expressing H1246 transformants fed with exogenous FFAs
| Strains | TAG (μg OD−1) | C18:2 | C18:3 | C20:5 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TAG (μg OD−1) | C18:2 in TAG (%) | PL (μg OD−1) | C18:2 in PL (%) | TAG (μg OD−1) | C18:3 in TAG (%) | PL (μg OD−1) | C18:3 in PL (%) | TAG (μg OD−1) | C20:5 in TAG (%) | PL (μg OD−1) | C20:5 in PL (%) | ||
| EV | 0.10 ± 0.01 | 0.21 ± 0.01 | – | 1.64 ± 0.02 | 21.85 ± 0.15 | 0.10 ± 0.01 | – | 1.87 ± 0.00 | 11.91 ± 0.87 | 0.14 ± 0.02 | – | 1.07 ± 0.09 | 2.90 ± 0.93 |
| CzDGAT1A | 1.09 ± 0.05 | 1.37 ± 0.05 | 11.51 ± 0.14 | 1.07 ± 0.12 | 5.51 ± 0.77 | 1.05 ± 0.02 | 7.18 ± 0.16 | 1.06 ± 0.04 | 3.05 ± 0.06 | 1.19 ± 0.02 | 22.39 ± 1.37 | 1.33 ± 0.01 | 4.68 ± 0.31 |
| CzDGAT1B | 0.24 ± 0.03 | 0.73 ± 0.01 | 12.20 ± 0.06 | 1.09 ± 0.01 | 12.02 ± 0.19 | 0.28 ± 0.01 | 19.91 ± 0.94 | 1.40 ± 0.04 | 15.16 ± 0.25 | 0.92 ± 0.01 | 14.15 ± 0.78 | 1.38 ± 0.01 | 3.86 ± 0.17 |
| CzDGTT1 | 0.11 ± 0.02 | 0.36 ± 0.14 | 15.60 ± 3.23 | 0.92 ± 0.01 | 14.04 ± 0.00 | 0.24 ± 0.05 | 26.79 ± 8.79 | 1.08 ± 0.05 | 9.24 ± 0.25 | 0.19 ± 0.04 | – | 1.12 ± 0.08 | 4.83 ± 0.14 |
| CzDGTT2 | 0.13 ± 0.04 | 0.13 ± 0.00 | – | 0.99 ± 0.01 | 16.37 ± 0.02 | 0.15 ± 0.00 | – | 1.24 ± 0.03 | 10.29 ± 0.42 | 0.50 ± 0.07 | 22.20 ± 0.79 | 1.21 ± 0.02 | 4.84 ± 0.05 |
| CzDGTT3 | 0.18 ± 0.02 | 0.31 ± 0.03 | – | 1.64 ± 0.04 | 24.44 ± 0.44 | 0.29 ± 0.00 | – | 2.22 ± 0.02 | 22.19 ± 0.24 | 0.24 ± 0.01 | – | 1.02 ± 0.00 | 2.62 ± 0.08 |
| CzDGTT4 | 0.10 ± 0.01 | 0.09 ± 0.01 | – | 1.13 ± 0.03 | 7.86 ± 0.34 | 0.20 ± 0.01 | – | 1.51 ± 0.09 | 23.49 ± 0.44 | 0.16 ± 0.03 | – | 1.20 ± 0.02 | 1.91 ± 0.19 |
| CzDGTT5 | 0.27 ± 0.02 | 0.43 ± 0.03 | 11.99 ± 0.25 | 1.03 ± 0.02 | 14.23 ± 0.13 | 0.27 ± 0.00 | 9.83 ± 0.11 | 1.30 ± 0.00 | 14.84 ± 0.30 | 0.35 ± 0.04 | 4.08 ± 0.23 | 1.21 ± 0.01 | 1.49 ± 0.38 |
| CzDGTT6 | 0.13 ± 0.02 | 0.08 ± 0.01 | – | 1.23 ± 0.04 | 16.09 ± 0.35 | 0.43 ± 0.03 | – | 1.68 ± 0.06 | 21.30 ± 0.04 | 0.18 ± 0.01 | – | 0.98 ± 0.00 | 0.86 ± 0.09 |
| CzDGTT7 | 0.19 ± 0.06 | 0.26 ± 0.01 | – | 1.13 ± 0.04 | 14.41 ± 0.02 | 0.15 ± 0.01 | – | 1.27 ± 0.06 | 8.71 ± 0.35 | 0.14 ± 0.01 | – | 1.00 ± 0.06 | 0.98 ± 0.34 |
| CzDGTT8 | 0.12 ± 0.01 | 0.19 ± 0.04 | – | 1.42 ± 0.06 | 24.91 ± 0.12 | 0.22 ± 0.02 | – | 1.79 ± 0.03 | 23.01 ± 0.48 | 0.16 ± 0.02 | – | 1.20 ± 0.14 | 0.50 ± 0.07 |
The data are expressed as mean ± SD (n = 3). PL polar lipids, FFAs free fatty acids, – under detectable level