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. 2019 Feb 11;19:136. doi: 10.1186/s12885-019-5337-6

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — a- Efficient cellular uptake of miR-200c carboxy-fluorescein-labeled nanoparticles. Confocal microscopy analysis of the cellular uptake of liposomes tagged with fluorescein 24 h after transfection with 100 nmol/l (A1) 95% of miR-200c enter KW-634 non-metastatic lung tumor cell line, (A2) miR-200c in the 821-LN metastatic tumor cell line, and (A3) miR-200c in the 821-T4 metastatic tumor cell line. b- Staining techniques showing cytoplasmic and nuclear carboxy-fluorescein-labeled incorporation of our Nano miR-200c and Red CellLight Mitochondria-RFP miR-200c migration in the 821-T4 metastatic tumor cell line at about 87.5%, allowing the observation of the mitochondrial response to our gene transfer