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. 2019 Feb 11;19:136. doi: 10.1186/s12885-019-5337-6

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 8 — Invasion (a) and Migration (b) studies in mouse lung non-metastatic KW-634 cells; invasion assay in metastatic 821-T4 cells (c), and migration in metastatic 821-LN cells (d) (blue control, green miR-200c treatment, red Nano miR-200c treatment). Protein assay using the ActivSignal IPAD Platform to help understand the pathway activation/inhibition profiles of cells by assessing the signaling pathways in each sample of mouse lung non-metastatic KW-634 cells (e). The IPAD assay allows direct measurement of the expression and modification of components of signaling pathways. Hippo pathway activity (e₁), Cell cycle regulation (e₂) and EMT (e₃)