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. 2019 Feb 11;20:125. doi: 10.1186/s12864-019-5501-z

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — qRT-PCR validation of differential expressed unigenes. a. The expression of nine randomly selected unigenes was analyzed by qRT-PCR. The data represent the means and standard errors from three biological replicates. b. Correlation analysis of the gene expression ratios calculated from the qRT-PCR and RNA-seq data. The qRT-PCR log2 values (y-axis) were plotted against the RNA-seq log2 values (x-axis). The asterisks on the correlation coefficient (R) indicates the highly significant difference at P <0.01