Table 1.

Numbers of WT and As3mt-KO mice in the LFD and HFD groups, with low or high folate intake and 0 or 100 ppb iAs in drinking water at the start and the end of the study, and numbers of mice used for phenotyping (FBG, FPI, body composition) and other analyses carried out in the course of the study.

	Week (n)	Genotype	Low folate		High folate
			$0\text{ppb}$ iAs	$100\text{ppb}$ iAs	$0\text{ppb}$ iAs	$100\text{ppb}$ iAs
Females
Start of study	0	WT	16	16	16	16
		KO	20	20	16	16
LFD, urine arsenic	6	WT	16	8	15	10
		KO	10	10	8	8
LFD, plasma folate	6	WT	7	7	8	8
		KO	9	10	8	8
LFD, FBG	24	WT	16	15	15	15
		KO	19	20	16	16
LFD, FPI	24	WT	16	14	15	15
		KO	19	20	16	16
LFD, body composition	24	WT	16	15	15	15
		KO	19	20	16	16
HFD, FBG	32	WT	16	15	15	15
		KO	19	20	16	16
HFD, FPI	32	WT	16	15	15	15
		KO	19	20	16	16
HFD, body composition	32	WT	16	15	15	15
		KO	19	20	16	16
HFD, urine arsenic	37	WT	14	16	14	13
		KO	14	10	8	10
HFD, plasma folate	37	WT	8	7	8	8
		KO	9	10	8	8
Liver arsenic	37	WT	12	10	14	14
		KO	14	8	14	8
End of study	37	WT	16	15	15	15
		KO	19	20	16	16
Total deaths	—	WT	0	1	1	1
		KO	1	0	0	0
Males
Start of study	0	WT	16	16	16	16
		KO	20	20	16	20
LFD, urine arsenic	6	WT	14	11	9	9
		KO	16	12	10	10
LFD, plasma folate	6	WT	7	8	6	7
		KO	10	8	7	11
LFD, FBG	24	WT	16	16	16	16
		KO	18	17	15	20
LFD, FPI	24	WT	16	16	15	16
		KO	18	17	15	20
LFD, body composition	24	WT	16	16	16	16
		KO	18	17	15	20
HFD, FBG	32	WT	16	16	16	16
		KO	18	16	14	20
HFD, FPI	32	WT	16	16	16	16
		KO	17	14	13	19
HFD, body composition	32	WT	16	16	16	16
		KO	18	16	14	20
HFD, urine arsenic	37	WT	12	16	10	16
		KO	10	12	10	13
HFD, plasma folate	37	WT	7	8	6	7
		KO	10	8	7	11
Liver arsenic	37	WT	10	11	10	9
		KO	9	12	8	14
End of study	37	WT	16	16	15	16
		KO	18	16	14	20
Total deaths	—	WT	0	0	1	0
		KO	2	4	2	0

Note: —, not applicable; FBG, fasting blood glucose; FPI, fasting plasma insulin; HFD, high-fat diet; iAs, inorganic arsenic; KO, arsenic ($+3$) methyltransferase knockout mice; LFD, low-fat diet; WT, wild type.