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Overview of different holding solutions[20]
| Medium | pH | Osmolarity | Buffer | Comments |
|---|---|---|---|---|
| Human Serum | 7.4 | 289 | Bicarbonate | Bicarbonate is a suboptimal pH buffer for cold storage solutions |
| Normal Saline | 5.0 | 308 | None | Extracellular balance |
| Ringer Lactate | 6.5 | 273 | Lactate | Extracellular balance |
| Plasma-lyte A | 7.4 | 294 | Acetate gluconate | Extracellular Balance |
| Hypothermosol- FRS | 7.6 | 360 | HEPES | Intracellular-like balance |
| Sterile water | 5.5 | 0 | None | If used will kill all grafts |
