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. 2019 Feb 5;10:32. doi: 10.3389/fgene.2019.00032

FIGURE 2.

FIGURE 2

A representation and interpretation of the results of PCR run II. (A) An electrophoresis gel, where in well 1 is a DNA ladder; 2 is the control (no DNA); 3, 4, and 6 show no amplification for tested SF adjacencies 8670637a and 8665179, 8667696a and 8667368, 8669739a and 8669686, respectively. Order of these SF was suggested by RACA; 5 exhibits amplification for adjacent SFs 8669889a and 8669162b in the order suggested by RACA. (B) A schematic representation and interpretation of the results form well 5 (SFs 8669889a and 8669162b were confirmed to be adjacent suggesting that the original scaffolds 8669889 and 8669162 these two SFs originate from were chimeric); (C) A schematic representation and interpretation of the results from well 3. The order of SFs 8670637a and 8665179, adjacent in RACA’s output was not confirmed due to no PCR amplification, suggesting that no conclusion about the chimeric nature of scaffolds 8670637 and 8665179 could be made.