KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| ZO-1 | Invitrogen | Cat#40-2200; RRID: AB_2533456 |
| Claudin-1 | Invitrogen | Cat#71-7800; RRID: AB_2533997 |
| PAK4 | Cell Signaling | Cat#3242S; RRID: AB_2158622 |
| phospho-MLC2 | Cell Signaling | Cat#3675S; RRID: AB_2250969 |
| MLC2 | Cell Signaling | Cat#3672S; RRID: AB_10692513 |
| FLAG-M2 | Sigma | Cat#F1804; RRID: AB_262044 |
| β-actin | Sigma | Cat#A2228; RRID: AB_746697 |
| Occludin | Life technologies | Cat#331-500; RRID: AB_2533101 |
| Rabbit IgG Alexa 488 | Life technologies | Cat#A24922; RRID: N/A |
| Mouse IgG Alexa 594 | Life technologies | Cat#A24921; RRID: AB_2536036 |
| Streptavidin, Alexa 488 | Life technologies | Cat#S32354; RRID: N/A |
| PDZ-RhoGEF(ARHGEF11) | BethylLaboratorues | Cat#A301-952A; RRID: AB_1548009 |
| RhoA | Cytoskeleton | Cat#ARH04; RRID: N/A |
| Esrp1 | (Warzecha et al., 2010) | N/A |
| Esrp2 | This paper | N/A |
| CD3 | Abcam | Cat#5690; RRID: AB_305055 |
| F4/80 | Abcam | Cat#6640; RRID: AB_1140040 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| EZ-Link sulfo-NHS-LC-biotin | Thermo Scientific | Cat#21327 |
| Lipofectamine 3000 | Invitrogen | Cat#11668019 |
| Bovine Serum Albumin | Cell Signaling | Cat#9998S |
| T4 DNA Ligase | Biolab | Cat#M0202S |
| 3X FLAG peptide | Sigma | Cat#F3290 |
| Protein A Agarose | Invitrogen | Cat#15918-014 |
| Random hexamers | Thermo | SO142 |
| M-MLV Reverse Transcriptase | Promega | M170A |
| Prolong Gold antifade reagent with DAPI | Invitrogen | Cat#P36935 |
| Anti-FLAG Affinity Gel | Sigma | Cat#A2220 |
| TransIT-293 | Mirus | Cat#MIR2700 |
| Critical Commercial Assays | ||
| Rho Activation Assay Biochem Kit | Cytoskeleton | Cat#BK036 |
| Millicell® ERS-2 Voltohmmeter | Millipore | Cat#MERS0002 |
| Experimental Models: Cell Lines | ||
| MKC-6 | (Troy and Turksen, 1999) | N/A |
| 293T | ATCC | CRL-3216 |
| Py2T | Gerhard Christofori (Waldmeier et al., 2012) | N/A |
| Experimental Models: Mice Strains | ||
| Esrp1−/−;Esrp2−/− | (Bebee et al., 2015) | N/A |
| Esrp1−/−;Esrp2+/+ | (Bebee et al., 2015) | N/A |
| Esrp1f/f;Esrp2−/−;K5-rtTA;tetO-Cre | This paper | N/A |
| Tg(tetO-cre)1Jaw/J (tetO-Cre) | The Jackson Laboratory | JAX stock #006224 |
| Keratin 5-rtTA (K5-rtTA) | Sarah Millar (Penn) | (Diamond et al., 2000) |
| Oligonucleotides | ||
| Primers for amplification of hEpi-ARHGEF11 cDNA for recombinant protein expression Forward: | This paper | N/A |
| GGGAATTCGCCACCATGAGTGTAAGGTTACCCCA | ||
| Primers for amplification of hEpi-ARHGEF11 cDNA for recombinant protein expression Reverse: | This paper | N/A |
| GGGCGGCCGCTGGTCCTGGTGACGCGGCTG | ||
| Primers for amplification of mEpi-Arhgef11 cDNA for recombinant protein expression Forward: | This paper | N/A |
| GGGAATTCGCCACCATGAGTGTAAGGTTACCCCA | ||
| Primers for amplification of mEpi-Arhgef11 cDNA for recombinant protein expression Reverse: | This paper | N/A |
| GGGCGGCCGCTGGTCCTGGTGACGCGGCTG | ||
| Arhgef11 and ARHGEF11 RT-PCR Forward: | This paper | N/A |
| TCAAGCTCAGAACCAGCAGGAAGT | ||
| Arhgef11 and ARHGEF11 RT-PCR Reverse: | This paper | N/A |
| TGCTCGATGGTGTGGAAGATCACA | ||
| Single-guide RNA Sequences, see Table S1 | This paper | N/A |
| PCR primer sequences used to confirm CRISPR/Cas9 mediated KO, see Table S2 | This paper | N/A |
| shRNA sequences used for Esrp1 or Esrp2 knock down experiments, see Table S3 | This paper | N/A |
| Recombinant DNA | ||
| psPAX2 | Addgene | Addgene#12260 |
| pCMV-VSV-G | Addgene | Addgene#8454 |
| Plasmid: pSg-Puro | This paper | N/A |
| Plasmid: pLKO.1-puro | Sigma | #SHC001 |
| Plasmid: pIBX-Epi-Arhgef11 | This paper | N/A |
| Plasmid: pIBX-Mes-Arhgef11 | This paper | N/A |
| Plasmid: pIPX-Epi-ARHGEF11 | This paper | N/A |
| Plasmid: pIPX Mes-ARHGEF11 | This paper | N/A |
| Plasmid: pIBX-Epi-ARHGEF11 | This paper | N/A |
| Plasmid: pIBX-Mes-ARHGEF11 | This paper | N/A |
| Plasmid: pIBX-Cas9 | This paper | N/A |
| Plasmid: PX330 | Addgene | Addgene#42230 |
| ARHGEF11 cDNA | Dharmacon | BC057394 |
| Arhgef11 cDNA | Dharmacon | BC079565 |
| Plasmid: pMG1-SVT | (Eyckerman et al., 2001) | N/A |
| Plasmid: pSEL+2L-hMal | (Eyckerman et al., 2001) | N/A |
| Plasmid: pMG1-GW | (Eyckerman et al., 2001) | N/A |
| Plasmid: pSEL+2L-GW | (Eyckerman et al., 2001) | N/A |
| Plasmid: pXP2d2-rPAP1-luci | (Eyckerman et al., 2001) | N/A |
| Software and Algorithms | ||
| Prizm | Graphpad | N/A |
| ImageQuant TL, version 7.0 | GE Healthcare | N/A |