Fig. 2.

Outline of the experimental protocols. Four groups of 2-d-old piglets were assigned to 1 of 4 diets fed at 4-h intervals for 9 (n = 24) and 21 d (n = 22). Catheters were placed surgically in the left jugular vein and stomach on d 0 of the study. Quantitative assessment of fat and lean body composition was performed using dual-energy X-ray absorptiometry on d −1 and 18. Body weights were recorded every 3 d. Blood levels of circulating insulin, glucose, and free AA were measured on d 9 and 21 at 0, 30, 60, 90, 120, 180, and 240 min post-feeding. Protein synthesis rate was measured on d 9 and 21, followed by euthanasia and tissue collection.