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. 2019 Jan 28;11(2):169–186. doi: 10.2217/epi-2018-0150

Figure 3. . ANGPTL8 exhibits intragenic enhancer chromatin overlapping DNA hypomethylation specifically in liver, which has the highest expression.

Figure 3. 

(A) ANGPTL8 is embedded in intron 14 of 47 introns of DOCK6 (chr19:11,339,832–11,363,081). (B–D) Predicted chromatin states, TF binding sites and DNA methylation profile from bisulfite-seq as in Figure 2. Dotted boxes in (B) and (C) indicate regions where liver-associated DNA hypomethylation was seen. (E) DNA methylation profiling by RRBS (with an 11-color gradient) including cell cultures for which bisulfite-seq DNA are not available. (F) and (G), RNA seq data as in Figure 2. MCF-7 and LNCaP, breast and prostate cancer cell lines, respectively; blue highlighting in panel (C), two neighboring RXRA sites and a HNF4A site that overlaps one of them.

ESC: Embryonic stem cell; Expr: Expression; fib: Fibroblast; HepG2: Hepatocarcinoma cell; HMEC: Human mammary epithelial cell; HUVEC: Human umbilical vein endothelial cell; K562: Myelogenous leukemia cells; LCL: Lymphoblastoid cell line; Myob: Myoblast; PBMC: Peripheral blood mononuclear cells; RRBS: Reduced representation bisulfite sequencing; Sk muscle: Skeletal muscle; TF: Transcription factor.