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. Author manuscript; available in PMC: 2020 Jan 29.
Published in final edited form as: Circulation. 2019 Jan 29;139(5):679–693. doi: 10.1161/CIRCULATIONAHA.118.034615

Figure 6. The LMO7 LIM domain is required for interaction with c-FOS and c-JUN and regulation of ECM and TGF-β pathway genes.

Figure 6

(A) HEK293A cells were transfected with pcDNA3-mLmo7-FL or ΔC and c-FOS (left panel) or c-JUN (right panel) plasmids. After 24 hrs, cell lysates were harvested for immunoprecipitation with LMO7 antibody and western analysis. The image shown is representative of two independent experiments. (B, C) (B) qPCR (n=4 independent experiments) or (C) Western analysis of Lmo7+/+ or Lmo7−/− mouse SMCs transfected with pcDNA3 vector, pcDNA3-mLmo7-FL or ΔC for 24 hrs as indicated. Quantification of c-FOS, c-JUN and β3 integrin expression and SMAD3 phosphorylation in western is shown on right (n=3 independent experiments). One-way ANOVA and multiple comparison with Sidak correction analysis revealed significant differences between conditions as indicated by asterisks in panels (B-C). Data are expressed as mean ± S.E.M. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.