Improved glucose clearance in HD/HCD-fed Liv-Lipa−/− mice. GTT and ITT were performed in female control and Liv-Lipa−/− mice fed HFHCD for 10 and 12 weeks, respectively. Plasma glucose concentrations after i.p. injection of (A) glucose (2 g/kg) and (B) insulin (0.25 U/kg), respectively (n = 8). Hepatic mRNA expression of genes involved in (C) gluconeogenesis and (D) tricarboxylic acid cycle genes relative to cyclophilin A as reference gene in female mice fed a HF/HCD for 20 weeks (n = 3–7). (E) Plasma β-hydroxybuyrate concentrations in male mice fed HF/HCD for 10 weeks (n = 5–7). Data represent mean ± SD; p < 0.05 (*), p ≤ 0.01 (**), p ≤ 0.001 (***). (A, B) ANOVA. (C–E) Student's unpaired t-test.