KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies (clone) | ||
| α4β7-APC (A4B7) | http://nhpreagents.org/NHP/default.aspx | Cat# PR-1421 |
| CD3-BV786 (SP34.2) | 3D Biosciences | Cat# 563918; RRID: AB_2738487 |
| CD8-BV650 (RPA-T8) | 3D Biosciences | Cat# 563822; RRID: AB_2744462 |
| CD8-BV605 (SK1) | BD Biosciences | Cat# 564116; RRID: AB_2744466 |
| CD14-BUV395 (MϕP9) | BD Biosciences | Cat# 563561; RRID: AB_2744288 |
| CD16-BUV496 (3G8) | BD Biosciences | Cat# 564653; RRID: AB_2744294 |
| CD20-BUV395 (L27) | BD Biosciences | Cat# 740204; RRID: AB_2739954 |
| CD56-BV786 (NCAM16.2) | BD Biosciences | Cat# 564058; RRID: AB_2738569 |
| CD62L-BV711 (SK11) | BD Biosciences | Cat# 565040; RRID: AB_2744438 |
| CD69-ECD (TP1.55.3) | Beckman Coulter | Cat# 6607110; RRID: AB_1575978 |
| CD95/Fas-BUV737 (DX2) | BD Biosciences | Cat# 564710; RRID: AB_2738907 |
| CD95/Fas-APC (DX2) | BD Biosciences | Cat# 558814; RRID: AB_398659 |
| CD107a/LAMP-1-PECF594 (H4A3) | BD Biosciences | Cat# 562628; RRID: AB_2737686 |
| CD107a/LAMP-1-PE-Cy7 (H4A3) | BD Biosciences | Cat# 561348; RRID: AB_10644018 |
| CD159a/NKG2a-APC (Z199) | Beckman Coulter | Cat# A60797; RRID: AB_10643105 |
| CD159a/NKG2a-PC7 (Z199) | Beckman Coulter | Cat# B10246; RRID: AB_2687887 |
| CD197/CCR7-BV421 (G043H7) | BioLegend | Cat# 353208; RRID: AB_11203894 |
| CD247/CD3ζ-FITC (H146-968) | ThermoFisher Scientific | Cat# MA5-17673; RRID: AB_2539063 |
| CD328/Siglec-7-PerCP-Vio700 (REA214) | MILTENYI | Cat# 130-100-979; RRID: AB_2657543 |
| CD335/NKp46-PC5 (BAB281) | Beckman Coulter | Cat# A66904 |
| CD335/NKp46-PC7 (BAB281) | Beckman Coulter | Cat# B38703 |
| CD335/NKp46-PE (BAB281) | Beckman Coulter | Cat# IM3711; RRID: AB_1575960 |
| CD337/NKp30-BV605 (p30-15) | BD Biosciences | Cat# 563384; RRID: AB_2738170 |
| DAP12-Alexa Fluor488 (406288) | NOVUS BIOLOGICALS | Cat# IC5240G |
| EOMES-PerCP-eFluor710 (WD1928) | ThermoFisher Scientific | Cat# 46-4877-42; RRID: AB_2573759 |
| FcR (γ-chain)-FITC (rabbit polyclonal) | Millipore Milli-Mark | Cat# FCABS400F; RRID: AB_11203492 |
| FcR (γ-chain)-Alexa Fluor700 (rabbit polyclonal) | Millipore Milli-Mark | Conjugated in-house |
| Granzyme B-Alexa Fluor700 (GB11) | BD Biosciences | Cat# 560213; RRID: AB_1645453 |
| HELIOS-e450 (22F6) | ThermoFisher Scientific | Cat# 48-9883-42; RRID: AB_2574136 |
| HELIOS-PE E610 (22F6) | ThermoFisher Scientific | Cat# 61-9883-42; RRID: AB_2574682 |
| HLA-DR-ECD (HLA-DR) | Beckman Coulter | Cat# IM3636; RRID: AB_10643231 |
| IFN-γ-PE-Cy7 (B27) | BD Biosciences | Cat# 560924; RRID: AB_2033978 |
| IFN-γ-BV711 (B27) | BD Biosciences | Cat# 564039; RRID: AB_2738557 |
| MIP1-β-APC-H7 (D21-1351) | BD Biosciences | Cat# 561280; RRID: AB_10611567 |
| Perforin-FITC (pf344) | mABTech | Cat# 3465-7; RRID: AB_1925742 |
| Perforin-Pacific Blue (dG9) | BioLegend | Cat# 308118; RRID: AB_10899565 |
| phospho-CD3ζ-Alexa Fluor647 (K25-407.69) | BD Biosciences | Cat# 558489; RRID: AB_647152 |
| phospho-Syk-Pacific Blue (rabbit polyclonal) | Cell Signaling Technology | Cat# 12967 |
| phospho-Syk/ZAP70-PE-Cy7 (17A/P-ZAP70) | BD Biosciences | Cat# 561458; RRID: AB_10696417 |
| Syk-PE (4D10.1) | ThermoFisher Scientific | Cat# 17-6696-42; RRID: AB_10714836 |
| TNF-α-BV650 (Mab11) | BD Biosciences | Cat# 563418; RRID: AB_2738194 |
| ZAP70-Pacific Blue (1E7.2) | ThermoFisher Scientific | Cat# MHZAP7004-4; RRID: AB_2539777 |
| Critical Commercial Reagents | ||
| Fix Buffer I phosflow™ | BD Biosciences | Cat# 557870 |
| Perm Buffer III phosflow™ | BD Biosciences | Cat# 558050 |
| Fixation Medium A | ThermoFisher Scientific | Cat# GAS001S100 |
| Permeabilization Medium B | ThermoFisher Scientific | Cat# GAS002S100 |
| Experimental Models: Organisms | ||
| Macaca mulatta | Tulane National Primate Research Center | N/A |
| Macaca mulatta | Alphagenesis Inc. | N/A |
| Software | ||
| Graphpad Prism 7 | GraphPad Software | https://www.graphpad.com/scientific-software/prism/ |
| FacsDiva | BD Biosciences | http://www.bdbiosciences.com/us/instruments/clinical/software/flowcytometry-acquisition/bd-facsdiva-software/m/333333/overview |
| FlowJo | FlowJo LLC | https://www.flowjo.com/solutions/flowio/downloads |