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. Author manuscript; available in PMC: 2019 Feb 12.
Published in final edited form as: J Control Release. 2017 May 30;260:100–110. doi: 10.1016/j.jconrel.2017.05.032

Fig. 4.

Fig. 4.

Acid does not trigger Dox release from AF488-labeled CP-ami-Dox/CP-BHQ2 mixed micelles (ami: amide linker) in live cells. (A) No Dox is released from CP-ami-Dox/CP-BHQ2 mixed micelles in aqueous solution at pH4 or pH7 in sodium phosphate buffer as a function of time post-incubation. (B) Average AF488 intensity in endo-lysosomes as a function of pH over time. Endo-lysosomal distribution of AF488-labeled CP is invariant across all pH but increases with time. (C) Average AF488 intensity in endo-lysosomes, cytosol and nuclei over time. AF488-labeled CP accumulates in endo-lysosomes with linear kinetics, but does not traffic to the cytosol and nuclei. (D) Average Dox intensity in endo-lysosomes as a function of pH over time. Dox fluorescence is invariant across all pH and does not change over time. (E) Average Dox intensity in endo-lysosomes, cytosol and nuclei over time. Because Dox is not released from micelles, Dox fluorescence does not change inside or outside endo-lysosomes. (F) Dox/AF488 intensity ratio within the entire cell and within endo-lysosomes over time. Increased AF488 fluorescence and unchanged Dox fluorescence within cells and endo-lysosomes result in decreased Dox/AF488 intensity ratio over time.