Figure 5.

Application of PAmCherry and Halo tag labels for single-molecule tracking of MukB. All data recorded at 15.48 ms frame⁻¹. (a) Distributions of the apparent diffusion coefficient for MukB-PAmCherry and MukB-Halo-TMR. (b) Localisations and tracks of MukB-PAmCherry and MukB-Halo-TMR. Track colours correspond to the apparent diffusion coefficient. Scale bars 1 µm. (c) Track length distributions at 0.2 kW cm^–2 561 nm excitation intensity for MukB labelled with PAmCherry, Halo-TMR, and Halo-JF549. (d) Mean track length as a function of 561 nm excitation intensity. Error bars are  ±SEM from 3 repeats.