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. 2019 Feb 12;14(2):e0211652. doi: 10.1371/journal.pone.0211652

Fig 3. Characterization of FoxP mutants and RNAi lines.

Fig 3

(A) Schematic representation of the FoxP genomic region and extension of the FoxP deletion. The transcriptional start site is indicated with an arrow, the two alternative stop sites with red lines. The original location of the P-element insertion GS22100 is depicted with a black triangle. The FoxP sequence targeted by RNAi1 and RNAi2 is depicted as a blue line. Genes flanking FoxP on each side are also indicated. All three genes are oriented in the same direction. (B) Adult brain hemisphere of wildtype (Wt), FoxP null (FoxP-/-), w, UAS-Dcr2/Y; Actin-GAL4/+; UAS-FoxP-RNAi1 (FoxP-RNAi1)/+ and w, UAS-Dcr2/Y; Actin-GAL4/+; UAS-FoxP-RNAi2/+ (FoxP-RNAi2) stained with anti-FoxP antibody. Scale bar corresponds to 50 μm. Images were obtained from male brains at 0–2 hours post-eclosion.