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. 2018 Aug 31;51(44):443001. doi: 10.1088/1361-6463/aad055

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© 2018 IOP Publishing Ltd

Original content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI.

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Figure 3. — ‘ColorEM’ using elemental analysis by energy dispersive x-ray imaging. ColorEM: label-free (P), paint (Os) and labeling DNA (Au) and peptides (Cd) is compatible. (a) Part of an islet of Langerhans immuno-labeled for structures in DNA (10 nm gold) and insulin (QD). (b) Overlay image of Au (red), Cd (green), Os (yellow) and P (blue) allows identification of G4 structures (gold labels) and insulin (Cd). Note the localization of Au to heterochromatin enriched in P, whereas the Cd signal is enclosed within a combination of Os rings and P that likely identifies phospholipid membranes of the vesicles. Large scale data and full resolution data is available via www.nanotomy.org; Reproduced from [22]. CC BY 4.0.