Figure 4.
Overlay of fluorescence signal of mVenus-labelled EphA2 protein imaged by conventional FM (top) and super-resolution single molecule localization microscopy (SMLM) (bottom) with a TEM image in a freeze substituted and resin embedded HEK293T cell using a dedicated super-resolution CLEM protocol [34]. The structural resolution of in-resin SMLM was approx. 50 nm with an average single molecule localization accuracy of 17 nm. Reproduced from [29]. CC BY 4.0.