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. 2018 Aug 31;51(44):443001. doi: 10.1088/1361-6463/aad055

Figure 13.

Figure 13.

(a) HS-AFM images of α3β3 subcomplex of F1-ATPase undergoing conformational changes in the presence of adenosine triphosphate (ATP). The height of a nucleotide-free β subunit is larger than those containing ATP or adenosine diphosphate. The highest pixel positions marked with red dots shift counterclockwise. At 1.44 s, the α subunit adjacent to the nucleotide-free β subunit appears higher than the β subunit. Imaging rate, 12.5 fps. (b) HS-AFM images showing spiral filament formation by polymerization of the ESCRT-III protein Snf7 on a supported lipid membrane. Imaging rate, 3 fps. (c), (d) Simultaneously captured HS-AFM (c) and total internal reflection fluorescence microscopy (d) images of Cy3-labeled chitinase A (arrows) moving unidirectionally on a chitin microfibril. Imaging rate, 3 fps.