Table 1.

Approaches for CLEM and ColorEM.

	In	Detected	Oppertunity	Limitation
FLM	Photons	Visible photons	Live cells, large area	Needs probes, resolutiona
EM	Electrons	Electrons	Ultrastructure, unbiased	Limited probes, grey scale
CL	Electrons	Visible photons	HIGH resolution	Needs development
EDX	Electrons	X-rays	Endogenous and probes	Undefined molecule
EELS	Electrons	Electrons	Endogenous and probes	Undefined molecule
nanoSIMS	Ions	‘Molecules’	Endogenous andprobes	Resolution

^aExcept for nanoscopy; see text for details.