Table 1. Primers used in this study.
| Gene Name | Sequence 5’-3’ | Type | Amplicon length (bp) | Accession number |
|---|---|---|---|---|
| GAPDH | 5:TTCGTCATGGGTGTGAACCA | housekeeping | 142 | NM_002046.3 |
| 3:CTGTGGTCATGATGAGTCCTTCCA | ||||
| GUSB | 5:CTCATTTGGAATTTTGCCGAATTTTT | housekeeping | 81 | NM_000181.3 |
| 3:CCGAGTGAAGATCCCCTTTTTA | ||||
| YWHAZ | 5:TGGCTCGAGAATACAGAGAG | housekeeping | 99 | NM_001135699 |
| 3:GTGAAGCATTGGGGATCAAG | ||||
| SCX | 5: CAGCGGCACACGGCGAAC | Tendon | 163 | BK000280 |
| 3: CGTTGCCCAGGTGCGAGATG | ||||
| TNC | 5:CCACAATGGCAGATCCTTCT | Tendon | 118 | NM_002160 |
| 3: GTTAACGCCCTGACTGTGGT | ||||
| DCN | 5:CTCTGCTGTTGACAATGGCTCTCT | Tendon | 256 | NM_001920 |
| 3:TGGATGGCTGTATCTCCCAGTACT | ||||
| COMP | 5:AGAAGTCCTATCGTTGGTTCC | Tendon | 104 | NM_000095 |
| 3: CAAGACCACGTTGCTGTC | ||||
| COL1A1 | 5:CCAGAAGAACTGGTACATCAGCAA | Tendon | 70 | NM_000088.3 |
| 3: CGCCATACTCGAACTGGAATC | ||||
| COL3A1 | 5:GGGAACATCCTCCTTCAACA | Tendon | 183 | NM_000090.3 |
| 3:GCAGGGAACAACTTGATGGT | ||||
| MMP3 | 5:CTGTTGATTCTGCTGTTGAG | Tendon | 126 | NM_002422.4 |
| 3:AAGTCTCCATGTTCTCTAACTG | ||||
| MMP13 | 5: AAGACTTCCCAGGAATTGGTGA | Tendon | 126 | NM_002427.4 |
| 3: GGCATGACGCGAACAATACG | ||||
| TIMP2 | 5: ATCTCATTGCAGGAAAGGCCG | Tendon | 103 | NM_003255.4 |
| 3: AGGCTCTTCTTCTGGGTGGT | ||||
| KLF4 | 5: AAGAGTTCCCATCTCAAGGCACA | Stemness | 90 | NM_001314052.1 |
| 3: GGGCGAATTTCCATCCACAG | ||||
| NANOG | 5: CAACTGGCCGAAGAATAGCAATG | Stemness | 110 | NM_001297698.1 |
| 3: TGGTTGCTCCAGGTTGAATTGTT | ||||
| KI67 | 5: AGCAAGCACTTTGGAGAGCA | Proliferation | 89 | NM_001145966.1 |
| 3: CATTGTCCTCAGCCTTCTTTGG | ||||
| PCNA | 5: GTAGTAAAGATGCCTTCTGGTG | Proliferation | 189 | NM_002592.2 |
| 3: TCTCTATGGTAACAGCTTCCTC |
Abbreviations: F, Forward Primer; R, Reverse Primer