Figure 3. Competition binding analysis to hIR-B of venom insulins (n = 2 independent measurements, each comprising three technical replicates).

Results are expressed as a percentage of binding in the absence of competing ligand (%B/B₀). Plotted values represent means ± s.d. Curve fitting was performed using Prism seven using nonlinear regression (one-site) analysis. IC50 values indicate half-maximal inhibitory concentration and are provided on the right (95% confidence intervals (CI)). Plotted values are provided in Source File 2 (Figure 3—source data 1.IR-B.binding).