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Representative dose-response curves for GGTDTC with and without supplemental CuSO4 for a) prostate cancer LNCaP and b) prostate normal epithelial PWR-1E cell lines, as determined by a resazurin viability assay measured at 24 h. c) GGT activity (blue) measured by E-pNA colorimetric assay, and IC50 values of GGTDTC (red) across multiple cell lines in FBS-free medium with 1 μM CuSO4 for 24 h. Antiproliferative activity of GGTDTC correlates with levels of GGT enzyme activity.
