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Components of 20× assay mixtures
| Component | Concentration (μM) |
|---|---|
| Forward primer | 18 |
| Reverse primer | 18 |
| Reference probe (FAM) | 5 |
| HDR probe (FAM) | 5 |
| NHEJ probe(s) (HEX/VIC) | 5 (each, if multiple probes are used) |
| Dark probe (3′ phosphate is added) | 10 (depending on the assay) |
