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. Author manuscript; available in PMC: 2019 Feb 12.
Published in final edited form as: Methods Mol Biol. 2018;1768:349–362. doi: 10.1007/978-1-4939-7778-9_20

Table 3.

Two-step thermal cycling for ddPCR (all the steps ramped by 2 °C/s)

Step Temperature (°C) Duration and repeat
1 95 10 min
2 94 30 s
3 59 1 min, (repeat steps 2 and 3, 39 more times)
4 98 10 min
5 12 Hold