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Reagents for quantification of HDR and NHEJ in genomic DNA samples (X μl (100–150 ng) of a genomic DNA sample later)
| Reagent | Volume per well |
|---|---|
| ddPCR Supermix for Probes (no dUTP) | 12.5 μl |
| 20× assay mixture | 1.25 μl |
| Restriction enzyme of choice | 2–4 U |
| Water | Up to 25—X μl |
