Target	Primer type	Primer sequence	Amplicon
zmp-1	Forward	CCAGACCTAACCCAACATCC	Forward + Reverse = 2692 in wild type worms (no band); 386 in mutants; Internal Forward + Reverse = 452 in wild type worms
	Reverse	CCAAAGATTGTGCCCAACAG
	Internal Forward	CCGATTGTTGTGCAGTATCG
zmp-2	Forward	AAACCTTGCAGTTTGACGGAGC	Forward + Reverse = 846 in wild type worms, 556 in mutants; Internal Forward + Reverse = 611 in wild type worms
	Reverse	ATAATTCAGTGCCCGAGAGC
	Internal Forward	GAAGGCCGACGCTCTAATG
zmp-3	Forward	GGTCTTCCGATTTGTCAAAGG	Forward + Reverse = 495 in wild type worms, 197 in mutants; Internal Forward + Reverse primers = 364 in wild type
	Reverse	GCTTCCGGCTCCATAAATCG
	Internal Forward	CAGCACTTCGGACATCAAAA
zmp-4	Forward	TCGCTTCCAATCTGCATTCC	Forward + Reverse = 697 in wild-type worms, 259 in mutants; Forward + Internal Reverse= 240 in wild type worms
	Reverse	AAAGAACTAGAGGAGCCCTCG
	Internal Reverse	CTTGTGACTTGTATGCCCATTCC
zmp-5	Forward	TACAAACAGTACACGGGAGC	Forward + Reverse = 840 in wild-type worms, 687 in mutants; Internal Forward + Reverse = 700 in wild type worms
	Reverse	AGACTTGCACTCCCTATTGG
	Internal forward	GCGTCAGAACATCACATTTGG
zmp-6	Forward	CATCTTCTGCCATGCCATCC	Forward + Reverse = 991 in wild type worms, 600 in mutants; Internal Reverse + Forward = 320 in wild type worms, no band in worms with the deletion
	Reverse	AGAACCTACACTCAAAGAGAGC
	Internal Reverse	ACAGGGGGAGTTGGGTTAAT