Table 1:

Comparison of established DNA adduct detection methods with reviewed emerging fluorescence-based methods.

Established methods	Maximum Sensitivity	Total DNA required (μg)	Citations
HPLC-ECD/fluorescence	1 in 108	1–100	[1,2]
Immunoassay	1 in 108	1–200	[1,2]
Radio labeled compounds	1 in 108	1–100	[1,2]
32P-post-labelling	1 in 1010	1–10	[1,2]
LC-MS and GC-MS	1 in 108	1–100	[2,7]
AMS	1 in 1012	1–2000	[1,2]
Emerging methods	Maximum Sensitivity	Total DNA required (μg)	Citations
Strand break reporter	1 in 105*	NA	[19,23]
In vitro RADD	1 in 105*	0.1–1	[27–30]
In vivo RADD	ND	NA	[31]
Fluorescent modifiers	ND	0.1–1	[42,44]

^*estimated from Mbp measurements