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. Author manuscript; available in PMC: 2020 Feb 12.
Published in final edited form as: Biochemistry. 2018 Dec 27;58(6):665–678. doi: 10.1021/acs.biochem.8b00716

Figure 3: Methylated Product Formation Assay.

Figure 3:

PchF catalyzes methyl transfer from its natural co-substrate AdoMet to the synthesized substrate analog, HPToxTred-CO2Et. Steady-state Michaelis-Menten kinetics were performed by a discontinuous assay. Substrate analog (eluting at 20 min) and product analog (eluting at 21.6 min) were separated by a C18 column using HPLC.