. Author manuscript; available in PMC: 2020 Feb 12.

Published in final edited form as: Biochemistry. 2018 Dec 27;58(6):665–678. doi: 10.1021/acs.biochem.8b00716

Table 4:

Kinetic parameter comparison between AdoMet, SeAdoMet, and TeAdoMet

Enzyme	Substrate Analog	k_cat (Se/Te)AdoMet / AdoMet	k_cat/K_M (Se/Te)AdoMet / AdoMet
CFA ^a Synthase	SeAdoMet	1.8	2.8
CFA ^a Synthase	TeAdoMet	0.50	0.059
COMT ^a	SeAdoMet	1.1	1.2
COMT ^a	TeAdoMet	0.16	0.0089
SET8 ^b	SeAdoMet	1.9	1.9
PchF-FL	SeAdoMet	1.9	2.2
PchF-FL	TeAdoMet	0.047	0.0010
PchF-AMT	SeAdoMet	2.2	3.0
PchF-AMT	TeAdoMet	0.022	0.0012

Iwig, Grippe, McIntyre, Booker. Biochemistry, 2004, 43, 13510-13524.

Linscott, Kapilashrami, Wang, Senevirathne, Bothwell, Blum, Luo. Proc. Natl. Acad. Sci., 2016, 113, E8369-E8378.