Fig. 3.

Silencing of Hsp90ab1 inhibited cell proliferation, invasion, and migration in vitro. a Hsp90ab1 mRNA in BGC823 and MKN28 cells after shRNA-mediated knockdown of Hsp90ab1 was detected by real-time RT-PCR. b–d Down-regulation of Hsp90ab1 resulted in inhibiting the proliferation and DNA replication of BGC823 and MKN28, as detected by colony formation assays (b), EdU incorporation assay (c) and CCK-8 assays (d). e The invasion ability of GC cells was significantly reduced in Hsp90ab1-depleted cells compared with the control groups, as revealed by Matrigel invasion assays. f Down-regulation of Hsp90ab1 reduced the migration ability of GC cells, as determined by Scratch-wound-healing assays. The data was expressed as the mean ± SD and reproduced in three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001