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. 2019 Feb 6;9:8. doi: 10.3389/fcimb.2019.00008

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Copyright © 2019 Martins, Carvalho, Faustino, Martins and Santos.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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LDs ζ-potential analysis at different WNV C concentrations. Variation of ζ-potential (Δζ) values of LDs samples isolated with TEE KCl buffer (K⁺ buffer) (A) and TEE NaCl buffer (Na⁺ buffer) (B) in the absence and in the presence of distinct WNV C concentrations. ζ-potential values determined for LDs without trypsin preincubation (filled symbols) and LDs pre-incubated with trypsin (empty symbols). WNV C concentration axes are presented in logarithmic scale. Results are presented as mean ± standard error (SE). Experiments were performed in triplicate. Solid lines were obtained by fitting the experimental data using Equation 3. WNV C binds to LDs inducing a progressive increase in the surface charge of the LD-WNV C complex.