Table 1.
Specific primers used to amplify blaOXA-LIKE genes and MLST data.
| Gene | Primer sequences 5’–3’ | Amplified (bp) | PCR | Reference |
|---|---|---|---|---|
| blaOXA-23 | GATCGGATTGGAGAA CAGA | 501 | OXA | Hujer et al., 2006 |
| ATTTCTGACCGCATTTCCAT | ||||
| bla OXA-24 | GGTTAGTTGGCCCCCTTAAA | 246 | OXA | Hujer et al., 2006 |
| AGTTGACGCAAAAGGGGATT | ||||
| bla OXA-51 | TAATGCTTTGATCGGCCTTG | 353 | OXA | Hujer et al., 2006 |
| TGCATTGCACTTCATCTTGG | ||||
| bla OXA-58 | AAGTATTGGGGCTTGTGCTG | 453 | OXA | Hujer et al., 2006 |
| CCCCTCTGCGCTCTACATAC | ||||
| gltA F | AAT TTACAGTGGCACATTAGGTCCC | 722 | MLST | Bartual et al., 2005 |
| gltA R | GCAGAGATACCAGCAGAGATACACG | Amp/Seq | ||
| gyrB F | TGAAGG CGGCTTATCTGAGT | 594 | MLST | Bartual et al., 2005 |
| gyrB R | GCTGGGTCTTTTTCCTGACA | Amp/Seq | ||
| gdhB 1F | GCTACTTTTATGCAACAGAGCC | 774 | MLST Amp | Bartual et al., 2005 |
| gdh secF | ACCATGCTTTGTTATG | Seq | ||
| gdhB 775R | GTTGAGTTGGCGTATGTTGTGC | 774 | MLST Amp | Bartual et al., 2005 |
| gdh secR | GTTGGCGTATGTTGTGC | Seq | ||
| recA F | CCTGAATCTTCYGGTAAAAC | 425 | MLST | Bartual et al., 2005 |
| recA R | GTTTCTGGGCTGCCAAACATTAC | Amp/Seq | ||
| cpn60 F | GGTGCTCAACTTGTTCGTGA | 640 | MLST | Bartual et al., 2005 |
| cpn60 R | CACCGAAACCAGGAGCTTTA | Amp/Seq | ||
| gpi F | GAAATTTCCGGAGCTCACAA | 456 | MLST | Bartual et al., 2005 |
| gpi R | TCA GGA GCA ATACCCCACTC | Amp/Seq | ||
| rpoD F | ACC CGT GAA GGT GAA ATC AG | 672 | MLST | Bartual et al., 2005 |
| rpoD R | TTC AGC TGG AGC TTT AGC AAT | Amp/Seq | ||
OXA-23 (blaOXA-23), OXA-24 (blaOXA-24), OXA-51 (blaOXA-51), and OXA-58 (blaOXA-58). Citrate synthase (gltA), DNA gyrase subunit B (gyrB), glucose dehydrogenase B (gdhB), homologous recombination factor (recA), 60-kDa chaperonin (cpn60), glucose-6 phosphate isomerase (gpi); RNA polymerase sigma factor (rpoD). Amp, Amplification; Seq, sequencing.