Table 1.
Effects of the inhibitors of PI3K LY-294002 (3 μM), ERK-MAPK kinase PD-98059 (3 μM) and PKC GF-109203X (0.1 μM) on the sensitivity and maximal responses of the CaCl2 concentration-response curves in mesenteric arteries stimulated by PE (10 μM) in a Ca2+-free medium.
| [Ca2+]i (F340/F380) | Tension (Nm-1) | |||||
|---|---|---|---|---|---|---|
| pEC50 | Emax | n | pEC50 | Emax | n | |
| Control | 3.77 ± 0.06 | 0.34 ± 0.04 | 7 | 4.12 ± 0.09 | 4.73 ± 0.47 | 7 |
| + LY-294002 | 3.86 ± 0.04 | 0.25 ± 0.03∗ | 7 | 3.79 ± 0.09∗∗ | 4.81 ± 0.61 | 7 |
| Control | 3.85 ± 0.08 | 0.42 ± 0.06 | 7 | 3.78 ± 0.06 | 4.36 ± 0.33 | 7 |
| + PD-98059 | 3.71 ± 0.17 | 0.23 ± 0.06∗∗∗ | 7 | 3.73 ± 0.24 | 4.24 ± 0.32 | 7 |
| Control | 3.96 ± 0.10 | 0.33 ± 0.04 | 8 | 3.91 ± 0.12 | 5.97 ± 0.63 | 8 |
| + GF-109203X | 3.90 ± 0.13 | 0.25 ± 0.04∗ | 8 | 3.93 ± 0.14 | 4.81 ± 0.69∗∗ | 8 |
Data are means ± SEM; “n” number of arteries (one per animal) from Wistar rats. Results are expressed as absolute values, as the increases in the intracellular Ca2+ concentration [Ca2+]i (ratio F340/F380) and tension (Nm-1) elicited by PE or CaCl2. pEC50 is – log(EC50), EC50 being the concentration of agonist giving 50% of the maximum effect (Emax). Significant differences were analyzed by paired Student’s t-test. ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001 vs. control.