Skip to main content
. 2019 Feb 12;10(2):133. doi: 10.1038/s41419-019-1364-0

Fig. 3. ZKSCAN3 is required for autophagy inhibition induced by A30P α-synuclein.

Fig. 3

a ZKSCAN3 shRNA apparently inhibited ZKSCAN3 levels. b Representative immunoblots of lysates from midbrain dopaminergic neurons expressing A30P α-synuclein were infected with either a scramble (SCR) or ZKSCAN3 shRNA virus. Total protein extracts were immunoblotted for LC3, ZKSCAN3, TFEB or p62. c–g Shown is the densitometric quantification of corresponding protein levels described in b. h Midbrain dopaminergic neurons expressing A30P α-synuclein were infected with either a SCR or ZKSCAN3 shRNA virus. Then, neurons were treated with and without Bafilomycin A1 (BafA1, 10 nM) for 12 h. LC3-I/II levels were analyzed by immunoblotting. i The histogram plot shows the densitometric quantification of LC3-II levels. j Luciferase reporter assay for MAP1LC3B or GABARAPL2 constructs in midbrain dopaminergic neurons expressing A30P α-synuclein stably transfected either scramble (SCR) or ZKSCAN3-shRNA virus. Values are shown as mean ± SEM (n = 3). Immunoblots are representative of n = 3. *P < 0.05; **P < 0.01 by Student t-test. Abbreviations: shRNA, small hairpin RNA