Fig. 6. A30P α-synuclein promotes nuclear translocation of ZKSCAN3 and inhibits JNK phosphorylation.

a Immunoblot analysis of nuclear and cytoplasmic subcellular fractions from midbrain dopaminergic neurons transduced with A30P α-synuclein AAV or empty virus. b A30P α-synuclein apparently inhibited p-JNK levels. c Midbrain dopaminergic neurons were infected by A30P α-synuclein AAV or control virus. The cell lysates were harvested for immunoblotting with the indicated antibodies. A30P α-synuclein suppressed the phosphorylation of JNK signaling pathway, while the phosphorylation of ERK and p38 was not inhibited. This inhibitory effect was rescued by anisomycin treatment d. The results were quantified from three independent experiments. Values are presented as mean ± SEM, ^*P < 0.05; ^**P < 0.01