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. 2019 Feb 12;9:1861. doi: 10.1038/s41598-019-38539-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterisation of orthosteric and allosteric activators of human GPR84. Flp-In T-REx 293 cells harbouring a human GPR84-Gα_i2 fusion protein were either untreated (−) or treated with doxycycline (100 ng.ml⁻¹, 24 h) (+) to induce expression of the fusion construct. Membranes from these cells were then used to measure binding of [³⁵S]GTPγS in response to varying concentrations of 2-HTP (a) and either PSB-16671 or DIM (b). Equivalent studies measured the effects of various concentrations of PSB-16671 on the potency and efficacy of 2-HTP (c) or vice versa (d). Data represent means ± S.E.M. of combined data from experiments performed on 4 individual membrane preparations. See Table 1 for quantitative analysis.