Figure 5.

Expression and function of GPR84 in murine RAW264.7 cells. Expression of mRNA encoding GPR84 in RAW 264.7 cells was detected by RT-PCR and compared to amplification of GPR84 from mouse genomic DNA (a) (+RT = reverse transcribed, −RT = without reverse transcription, NTC = no template control). Detection of GAPDH was used as a control (predicted amplicon 110 bp). RAW 264.7 cells were treated with LPS (100 ng.ml⁻¹, 5 h) and membranes subsequently prepared. The ability of varying concentrations of 2-HTP and PSB-16671 to promote binding of [³⁵S]GTPγS were assessed (b), as were the effects of various concentrations of PSB-16671 on the potency and efficacy of 2-HTP (c) or vice versa (d). Data represent means ± S.E.M. of combined data from experiments performed on 5 (b), 4 (c) or 3 (d) individual membrane preparations. See Table 2 for quantitative analysis.