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. 2019 Feb 12;10:711. doi: 10.1038/s41467-019-08441-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — Assessments of how iATPSnFR^1.0 was affected by pH. a–d Effect of pH on the unbound and ATP bound fluorescence of the indicated constructs. e, f Comparison of the effect of ATP on the fluorescence of the indicated constructs at pH 6.4 and 7.4. The error bars represent the s.e.m. and in some cases are smaller than the symbols used for the mean. n numbers are provided in the figure panels and refer to the number of cells in panel (a), and number of independent evaluations in (b–f)