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. 2019 Feb 12;9:1798. doi: 10.1038/s41598-019-39321-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of the chosen promoters and lac promoter via qPCR analysis. Transcription of gfp gene under different promoters in P. mendocina NKU was quantified at different growth phases. 16S rDNA gene was used as internal reference. The relative transcription value of gfp gene under lac promoter was set as 1. Data represent the mean values ± standard deviations of triplicate measurements from three independent experiments. A Student’s t-test was performed between lac promoter and chosen promoters. * and ** indicate P < 0.05 and P < 0.01, respectively.