Table 2.
Strains and plasmids used in this study.
| Strains and plasmids | Description | Source or reference |
|---|---|---|
| Strains | ||
| Pseudomonas mendocina NKU | PHAMCL producing strain; CmR; preserved in our laboratory; carrying an in-frame deletion in the upp gene; starting strain for engineering | This laboratory |
| P. mendocina NKU pBBR | NKU derivative containing pBBR1MCS-2; CmR; KmR | This work |
| P. mendocina NKU-Plac | NKU derivative containing pBBR1MCS-gfp; CmR; KmR | This work |
| P. mendocina NKU-P4 | NKU derivative containing pBBR1MCS-P4-gfp; CmR; KmR | This work |
| P. mendocina NKU-P6 | NKU derivative containing pBBR1MCS-P6-gfp; CmR; KmR | This work |
| P. mendocina NKU-P9 | NKU derivative containing pBBR1MCS-P9-gfp; CmR; KmR | This work |
| P. mendocina NKU-P16 | NKU derivative containing pBBR1MCS-P16-gfp; CmR; KmR | This work |
| P. mendocina NKU-P17 | NKU derivative containing pBBR1MCS-P17-gfp; CmR; KmR | This work |
| P. mendocina NKU-P18 | NKU derivative containing pBBR1MCS-P18-gfp; CmR; KmR | This work |
| P. mendocina NKU-P20 | NKU derivative containing pBBR1MCS-P20-gfp; CmR; KmR | This work |
| P. mendocina NKU-P23 | NKU derivative containing pBBR1MCS-P23-gfp; CmR; KmR | This work |
| P. mendocina NKU-P25 | NKU derivative containing pBBR1MCS-P25-gfp; CmR; KmR | This work |
| P. mendocina NKU-P29 | NKU derivative containing pBBR1MCS-P29-gfp; CmR; KmR | This work |
| P. mendocina NKU-4C1 | NKU derivative carrying an P4 promoter insertion into the upstream of phaC1 gene | This work |
| P. mendocina NKU-6C1 | NKU derivative carrying an P6 promoter insertion into the upstream of phaC1 gene | This work |
| P. mendocina NKU-16C1 | NKU derivative carrying an P16 promoter insertion into the upstream of phaC1 gene | This work |
| P. mendocina NKU-∆phaZ-4C1 | NKU-4C1 derivative carrying an in-frame deletion in the phaZ gene | This work |
| P. mendocina NKU-∆phaZ-6C1 | NKU-6C1 derivative carrying an in-frame deletion in the phaZ gene | This work |
| P. mendocina NKU-∆phaZ-16C1 | NKU-16C1 derivative carrying an in-frame deletion in the phaZ gene | This work |
| P. mendocina NKU-∆phaZ | NKU derivative carrying an in-frame deletion in the phaZ gene | This work |
| Escherichia coli DH5α | F−, φ80dlacZΔM1, Δ(lacZYA-argF)U169, deoR, recA1, endA1, hsdR17(rk−, mk+), phoA, supE44, λ−thi-1, gyrA96, relA1 | This laboratory |
| E. coli S17-1 | recA; harbors the tra genes of plasmid RP4 in the chromosome; proA thi-1 | This laboratory |
| Plasmids | ||
| pBBR1MCS-2 | Broad host range; expression plasmid; Kanr; Plac, mob | 27 |
| pBBR1MCS-gfp | pBBR1MCS-2 derivative containing gfp gene | This work |
| pBBR1MCS-P4-gfp | pBBR1MCS-2 derivative containing P4 promoter and gfp gene | This work |
| pBBR1MCS-P6-gfp | pBBR1MCS-2 derivative containing P6 promoter and gfp gene | This work |
| pBBR1MCS-P9-gfp | pBBR1MCS-2 derivative containing P9 promoter and gfp gene | This work |
| pBBR1MCS-P16-gfp | pBBR1MCS-2 derivative containing P16 promoter and gfp gene | This work |
| pBBR1MCS-P17-gfp | pBBR1MCS-2 derivative containing P17 promoter and gfp gene | This work |
| pBBR1MCS-P18-gfp | pBBR1MCS-2 derivative containing P18 promoter and gfp gene | This work |
| pBBR1MCS-P20-gfp | pBBR1MCS-2 derivative containing P20 promoter and gfp gene | This work |
| pBBR1MCS-P23-gfp | pBBR1MCS-2 derivative containing P23 promoter and gfp gene | This work |
| pBBR1MCS-P25-gfp | pBBR1MCS-2 derivative containing P25 promoter and gfp gene | This work |
| pBBR1MCS-P29-gfp | pBBR1MCS-2 derivative containing P29 promoter and gfp gene | This work |
| pEX18Tc-upp | pEX18Tc derivative, carrying a copy of upp gene of P. mendocina NKU | This laboratory |
| pEX18Tc-ΔphaZ | pEX18Tc-upp derivative, carrying the up- and downstream regions of phaZ gene, used for deletion of the phaZ gene | This work |
| pEX18Tc-P4 | pEX18Tc-upp derivative, used for insertion of P4 promoter in front of phaC1 | This work |
| pEX18Tc-P6 | pEX18Tc-upp derivative, used for insertion of P6 promoter in front of phaC1 | This work |
| pEX18Tc-P16 | pEX18Tc-upp derivative, used for insertion of P16 promoter in front of phaC1 | This work |